The Women's Tennis Association said an appearance on Sunday by Chinese tennis star Peng Shuai did not address its concerns about her wellbeing.
Peng said on Sunday that she had never accused anyone of sexually assaulting her, and that a social media post she had made early last month had been misunderstood.
"We remain steadfast in our call for a full, fair and transparent investigation, without censorship, into her allegation of sexual assault, which is the issue that gave rise to our initial concern," the WTA said in a statement.
Peng's well-being became a matter of concern among the global tennis community and rights groups when she appeared to allege that a former Chinese vice premier, Zhang Gaoli, had sexually assaulted her in the past. After that post, she was absent from public view for nearly three weeks.
"First, I need to stress one point that is extremely important, I have never said or written that anyone has sexually assaulted me, I have to clearly stress this point," Peng said in the video posted by Lianhe Zaobao, a Singapore media outlet.
Peng's remarks on Sunday marked the first time she had addressed the matter on camera in public. She spoke on the sidelines of a cross-country skiing event in Shanghai that she attended.
She said that her post on Weibo, a Twitter-like social media site, which had been quickly removed, was a "private matter".
Peng, 35, said in the video that "people have many misunderstandings" about her Weibo post. She did not elaborate.
She also said that she had been living at home in Beijing without supervision. She did not mention Zhang.
The WTA, which early this month said it would suspend tournaments in China immediately due to concerns over the treatment of Peng and the safety of other players, continued to call for an investigation.
"It was again good to see Peng Shuai in a public setting and we certainly hope she is doing well," it said in a statement.
"As we have consistently stated, these appearances do not alleviate or address the WTA's significant concerns about her well-being and ability to communicate without censorship or coercion," the WTA said.
China has not directly commented on Peng's initial post, but said after the WTA's move to suspend tournaments in China that it "opposes the politicisation of sports".
Zhang has not commented on the matter.
At the Shanghai event on Sunday, Peng appeared on a fifth floor viewing balcony with athletes from various sports, including former NBA basketball star Yao Ming, and watched for about 20 minutes, according to the Lianhe Zaobao report.
She wore a black jacket with a China flag and a red T-shirt with the characters for China.
